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Sorted-samples-EBAIDuring the dark winter months, Markus has been sorting our benthic samples. The deeper we go in the lake, the fewer orders we find, from the left to the right in the figure. Also the abundance of organisms was lower in the samples collected deeper.





Sorted samples EBAI 2Diptera were abundant both in the shallow lake benthos and in the river benthos. River sample on the left, lake sample on the right in this figure. Chironomidae were the most abundant Diptera both in the lake and in the river benthos.





PCR resultsAfter we extracted the total DNA from the samples, we amplified the barcode section of CO1 gene using different primer sets. We carried out this work in the Hajibabaei lab at the Centre for Biodiversity Genomics, Canada. The amplifications were successful as seen in this figure where we have visualized the amplicons on agarose gel.







Results from Illumina MiSeqThe amplicons were sequenced using Illumina Miseq system in the Hajibabaei lab. The result looks like this on the computer screen. Now we need to collect the good quality sequences, identify them and see whether we can find answers to our questions!


The second field-sampling trip in EBAI was directed to the Lake Jonsvatn on September 28. We sampled a depth gradient (0.25 m, 2 m, 7.5 m and 15 m) for benthic invertebrates starting from Trondhjems Roklub, and eDNA from water from the Roklub pier. Also, adult flying insects were sampled with a net. Weather conditions were perfect during the second successful field trip, and in addition to the samples, we got nice pictures including the spectacular panorama photo on top of the page.


Erik is sampling water from the pier. Photo: Torbjørn Ekrem.
Relaxing day in the field (at least for some of us). Photo: Elisabeth Stur.
Captain Hårsaker leaving the field site (we'll be back). Photo: Torbjørn Ekrem.

Field filtering requires some patience. Photo: Elisabeth Stur.

The first field trip in EBAI was held August 10-11 and went to the Atna watershed and the Rondane Mountains. Three stations, Vollen, Dørålseter and Skranglehaugan, were sampled for benthic invertebrates while eDNA from water was sampled at Dørålseter. We also got to test our mobile water filtering setup for the first time. Very successful!

Karstein samples water from the Atna River. Photo Torbjørn Ekrem.
Torbjørn and Markus preserve a kick-sample.  Photo: Karstein Hårsaker.
Karstein gets a kick-sample from  Vidjedalsbekken at Skranglehaugan. Photo: Torbjørn Ekrem.
Mobile water filtering lab at Dørålseter. Photo: Torbjørn Ekrem.