The second field-sampling trip in EBAI was directed to the Lake Jonsvatn on September 28. We sampled a depth gradient (0.25 m, 2 m, 7.5 m and 15 m) for benthic invertebrates starting from Trondhjems Roklub, and eDNA from water from the Roklub pier. Also, adult flying insects were sampled with a net. Weather conditions were perfect during the second successful field trip, and in addition to the samples, we got nice pictures including the spectacular panorama photo on top of the page.