{"id":246,"date":"2017-09-15T13:35:46","date_gmt":"2017-09-15T11:35:46","guid":{"rendered":"http:\/\/blogg.vm.ntnu.no\/ebai\/?p=246"},"modified":"2018-04-03T11:43:05","modified_gmt":"2018-04-03T09:43:05","slug":"learning-dna-metabarcoding","status":"publish","type":"post","link":"https:\/\/blogg.vm.ntnu.no\/ebai\/2017\/09\/15\/learning-dna-metabarcoding\/","title":{"rendered":"Learning DNA metabarcoding"},"content":{"rendered":"

One of the goals of the EBAI project is to distribute knowledge on DNA metabarcoding. We started with a small workshop with two students: Zhenhua Sun from Chalmers University, Sweden and Xiaolong Lin from NTNU University Museum. Our goal was to go through the steps of sample processing using own datasets.<\/p>\n

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To have a proper feeling on the methodology, we went to sample some water for eDNA analyses in Theissendammen. A suitable outfit for this kind of near-city sampling is a smart casual. (Photos Xiaolong Lin)<\/p>\n

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We expect to find mallard DNA in our samples. Also beaver is quite potential finding as Markus steps over a beaver dinner to go for the sample. (Photos Xiaolong Lin and Erik Bostr\u00f6m)<\/p>\n

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We visited the molecular lab and Markus showed how to extract DNA and amplify your target gene in PCR as well as explained the paired-end sequencing. (Photos Xiaolong Lin)<\/p>\n

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Using our own laptops, we analyzed our data. (Photo Xiaolong Lin)<\/p>\n","protected":false},"excerpt":{"rendered":"

One of the goals of the EBAI project is to distribute knowledge on DNA metabarcoding. We started with a small workshop with two students: Zhenhua Sun from Chalmers University, Sweden and Xiaolong Lin from NTNU University Museum. Our goal was to go through the steps of sample processing using own datasets. To have a proper ...continue reading \"Learning DNA metabarcoding\"<\/span><\/a><\/p>\n","protected":false},"author":99,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[3],"tags":[],"class_list":{"0":"post-246","1":"post","2":"type-post","3":"status-publish","4":"format-standard","6":"category-whats-new","7":"h-entry","8":"hentry","9":"h-as-article"},"_links":{"self":[{"href":"https:\/\/blogg.vm.ntnu.no\/ebai\/wp-json\/wp\/v2\/posts\/246","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blogg.vm.ntnu.no\/ebai\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blogg.vm.ntnu.no\/ebai\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blogg.vm.ntnu.no\/ebai\/wp-json\/wp\/v2\/users\/99"}],"replies":[{"embeddable":true,"href":"https:\/\/blogg.vm.ntnu.no\/ebai\/wp-json\/wp\/v2\/comments?post=246"}],"version-history":[{"count":7,"href":"https:\/\/blogg.vm.ntnu.no\/ebai\/wp-json\/wp\/v2\/posts\/246\/revisions"}],"predecessor-version":[{"id":263,"href":"https:\/\/blogg.vm.ntnu.no\/ebai\/wp-json\/wp\/v2\/posts\/246\/revisions\/263"}],"wp:attachment":[{"href":"https:\/\/blogg.vm.ntnu.no\/ebai\/wp-json\/wp\/v2\/media?parent=246"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blogg.vm.ntnu.no\/ebai\/wp-json\/wp\/v2\/categories?post=246"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blogg.vm.ntnu.no\/ebai\/wp-json\/wp\/v2\/tags?post=246"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}